DNA Microarray Core Facility

Upcoming Seminars & Events

Erythropoietin and Solid Tumorigenesis
Dr. William Kim (UNC Chapel Hill Lineberger Comprehensive Cancer Center)
Monday,April 30 2012, 1:00pm-2:00pm, 2240 CIEMAS Building

Therapeutic Implications of the Molecular Portraits of Breast Cancer
Dr. Charles Perou (UNC Chapel Hill Lineberger Comprehensive Cancer Center)
Monday,May 7 2012, 1:00pm-2:00pm, 2240 CIEMAS Building

Discovering Target-oriented mechanisms of action
GeneGO Training Series

One of the most common approaches of elucidating the effects of a gene or compound is to directly target this entity with various biochemical techniques including introducing competitive compounds and knocking down expression levels. As a consequence we are left with an observed phenotype with a gap in knowing how this phenotype arose. In this session we will demonstrate how to determine potential signaling mechanisms emanating from a ¡°target¡± to a phenotype. We will specifically focus on exploiting the annotating interaction content, toxicology/disease/phenotypic ontologies and the shortest- path network build algorithm. Example questions you may ask when using this approach include:

• How do I identify the mechanisms that cause toxicity ¡°X¡± after targeting a receptor?
• What are the mechanisms involved in inducing disease ¡°X¡± after knock out/ over-expression of my favorite gene?
• If I target my favorite gene I observe and increase in expression of several others. What are the mechanisms involve?
• My drug induced cardiotoxicity and this may be an off target effect. Can I determine the molecular basis of this observation?
• How to export search results

Tuesday, May 8 2012, 1:00pm-2:00pm, Click here to Register

Roles for Polyploidy in De-stabilizing the Genome
Dr. Donald Fox
Monday,May 14 2012, 1:00pm-2:00pm, 2240 CIEMAS Building

Agilent's Comprehensive Cancer Center Tour stop at Duke

Agenda:

• 9:30 - 9:45 am: Welcome and Overview
• 9:45 - 10:30 am: Discover and Design Your Cancer Gene Panel with NGS Target Enrichment Strategies
  Jennifer Carter, Field Application Scientist, Agilent Technologies
• 10:30 - 11:15 am: The Importance of Elucidating Epigenetic Profiles of Cancer Samples
  Alex Siebold, Field Application Scientist, Agilent Technologies
• 11:15 am - 12:00 pm: High-Resolution Detection of Copy Number Aberrations and Loss of Heterozygosity in Challenging Cancer Samples
• 12:00 pm: Lunch

Wednesday, May 16 2012, 9:00am-12:00pm,Duke University Medical Center MSRB Room 001 View Abastract and Register Here

Pathway Analysis of Serological Metabolite Profiles in Rheumatoid Arthritis

GeneGO case study series

Metabolomic data sets obtained using Chenomx NMR Suite often require additional analysis to place them in proper context. The GeneGo pathway analysis platform can help establish this context. In this tutorial we present an analysis using serological metabolic profiles from Chenomx NMR Suite, known inflammatory markers from prior studies of the K/BxN mouse model and the GeneGo platform to generate a mechanistic hypothesis for the K/BxN mouse model and Rheumatoid arthritis (RA). The basic workflow includes the following steps:

• Use Chenomx NMR Suite to obtain serological metabolite profiles using targeted profiling
• Integrate known inflammatory markers from previous studies of the mouse model system
• Use the GeneGo pathway analysis platform to generate a mechanistic hypothesis for the K/BxN mouse model

Monday, May 21 2012, 1:00pm-2:00pm, Click here to Register

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