DNA Microarray Core Facility

Affymetrix® GeneChip® Microarrays

Our Affymetrix GeneChip service includes:
- RNA Quality check:
  Total RNA concentration and purity check with NanoDrop
  Total RNA integrity check with Agilent Bioanalyzer
  Total RNA quality check summary report
- Affymetrix GeneChip microarray processing:
  cDNA synthesis, in vitro transcription, aRNA purification and fragmentation
  GeneChip array hybridization
  GeneChip array washing, staining and scanning
- Data processing and report:
  GeneChip array post scanning data processing
  Online data storage of GeneChip array data files
  GeneChip array scanning QC report
  Description of methods for microarray processing for manuscript
Contact microarraylab@duke.edu or call (919)668-1583 for pricing
- Discounted pricing is available to qualified investigators, click here to see requirements for discounted services.

The raw data files for miRNA arrays will be returned to your Express Repository Project ID folder via the web. No QC or summarized data will be returned. To generate summarized data, please download the appropriate miRNA QC tool software. The Affymetrix® miRNA QCTool enables a variety of analysis methods tailored to miRNA studies and features extensive quality control tools. Several methods of probe signal background subtraction, array normalization (including the use of internal normalization controls), and probe set summarization are allowed, in various combinations. If you are using internal normalization controls, you can apply the default probe sets (human 5.8S rRNA) or choose from any set of the probe sets on the array, including snoRNAs, miRNAs (any species), or spike-in controls. The miRNA QCTool is thus applicable to a widevariety of experimental designs.

Investigators that wish to use Affymetrix analysis should use our online submission forms to initiate the process.

(Holly Dressman discloses that she has equity in Expression Analysis)

For gene expression services not provided by Duke Microarray Facility, one can utilize Expression Analysis Institute. Pricing for services offered by Expression Analysis Institute can be obtained by going to the web site, and submit a request for a quote. For further questions about services at EAI and billing questions, please contact:
STEVE SIFERD SENIOR SERVICE CONSULTANT, SOUTHEAST,
PH 919-405-2248 | FX 919-806-0219 | SSIFERD@EXPRESSIONANALYSIS.COM
When submitting samples to Expression Analysis Institute for microarray services, please fill out our online submission form. Please use a Duke Fund code when prompted for charge code. This will be used for the Duke EAI IT set up fee. One will also need to provide EAI with a purchasing order number, this can be provided in the Notes section of the online submission form, and billing for services performed at EAI will need to be coordinated with Steve Siferd directly.