Duke Proteomics Core Facility

Instrumentation

Instrumentation

3100 OFFGEL Fractionator

3100 OFFGEL Fractionator
3100 OFFGEL Fractionator

3100 OFFGEL Fractionator Material from Agilent Website

Protein fractionation, in-solution recovery

Prefractionation of proteins and peptides prior to LC/MS analysis can dramatically increase the number of proteins ultimately identified. The Agilent 3100 OFFGEL Fractionator uses a novel isoelectric focusing technique to achieve excellent pI-based fractionation. The resulting fractions are in solution, making recovery for LC/MS analysis much easier than with old-fashioned gels. Results are highly reproducible, and the Agilent 3100 OFFGEL Fractionator is now even easier to use with its new web-based user interface.

  • 3100 OFFGEL Fractionator State-of-the-art user interface for online control of each individual sample
  • pI-based OFFGEL fractionation with 0.1 – 0.6 pH resolution depending on consumable kit used
  • In-solution fraction recovery for easy transfer to LC/MS
  • Two power supplies allow simultaneous fractionation of two sample sets with broad differences in concentration
  • Up to 16 samples fractionated at the same time
  • µg to mg load capacity
  • Possible to run conventional in-gel IEF as well as OFFGEL fractionation
  • Preconfigured methods for OFFGEL and in-gel modes
  • Online current control for each individual sample
  • Simple, reproducible, and easy to use