Duke Proteomics Core Facility

Instrumentation

Instrumentation

Digilab ProGest Protein Digestion Station

Digilab ProGest
Digilab ProGest

(Digilab ProGest Material from Digilab Global Website)

The ProGest Protein Digestion Station is an automated system for in-gel and in-solution digestion of proteins in order to prepare samples for LC/MS and/or MALDI/TOF. All washing steps, reduction(with DTT) and alkylation (with iodoacetamide) steps, enzyme activation and addition, and thermal incubation cycles are automatically performed and controlled using a touch screen interface. The unit can also be controlled using a desktop PC with the user-friendly Methods Editor software.

ProGest Features:

  • 96-well format in-solution or in-gel digestion
  • Multiple digestion runs per day
  • Flow-through reaction block with heating stage
  • Innovative concentric dual needle design
  • Aspiration-free sample processing
  • Fully enclosed
  • Unit can stand alone with touchpad control, or be PC-controlled
  • Methods include silver destaining, trypsin digestion, acidic and organic extraction