Duke RNAi Screening Facility

Open Biosystems (Expression Arrest™) shRNA

siRNA Library

Request Clones: shRNA Clone Request System.

Hannon-Elledge human shRNA library

This whole-genome collection of small-hairpin RNAs (shRNAs) was developed by Greg Hannon (CSHL) and Stephen Elledge (Harvard) and colleagues, and is being distributed by Open Biosystems (Expression Arrest™). Based upon the pSM2 retroviral vector backbone, this expressed shRNA library was designed with features aimed at increased knockdown efficiency and versatility of use, including:

  • Retroviral backbone for potential transient, stable, and in vivo use.
  • MicroRNA30-based hairpin design for increased knockdown efficiency.
  • Molecular 'barcoding' for clone identification from pooled clone studies.
  • Mating-assisted recombination cloning (MAGIC) for rapid transfer of shRNA cassette to other vectors.

The RNAi core has acquired the Hannon-Elledge shRNA vector library for distribution to Duke investigators. This library includes ~62,500 unique shRNA constructs targeting the entire human genome (>25,000 genes with near-redundant coverage). Through the RNAi Facility, Duke investigators can quickly and easily select and obtain shRNA clones representing a gene or genes of interest to place in their functional assays.

Download human shRNA library information sheet (PDF file).

References:
Silva, JM et al (2005) "Second-generation shRNA libraries covering the mouse and human genomes" Nat Genetics 37:11, 1281-1288.

Paddison, P et al (2004) "A resource for large-scale RNA-interference-based screens in mammals". Nature 428, 427-431.

Paddison, P et al (2004) "Cloning of short hairpin RNAs for gene knockdown in mammalian cells" Nature Methods Vol 1:2, 163-167.

Cleary, M et al (2004) "Production of complex nucleic acid libraries using highly parallel in situ oligonucleotide synthesis" Nat Methods 1:3, 241-248.

Links to shRNA Library Resources:

  • More information about Open Biosystems Expression Arrest™ System.
  • Search Open Biosystems human shRNA library to identify clones.
  • RNAi Codex - searchable database of publicly available RNAi resources, including the Hannon-Elledge shRNA libraries.
  • RNAi Central - the Hannon RNAi laboratory at CSHL.