Genome Sequencing & Analysis Core Resource

Ion Torrent PGM

Ion Torrent PGM

Ion Torrent PGM

We offer sequencing services on the Ion Torrent PGM. This is a new sequencer that uses semiconductor chip technology. It simply sequences DNA by monitoring hydrogen ions released every time a nucleotide is incorporated (see Ion torrent page). No enzymatic cascade, no fluorescence, no chemiluminescence etc... In consequence, this sequencing technology is extremely fast (<3 hours) and relatively cheap. Three chips with different throughput are available to accomodate your project's needs: 314, 316, and 318 chips. At the moment we only provide DNA fragment library preparation services.We will soon provide cDNA and amplicon library preparation services.

Sample requirements

Samples must be resuspended in TE or nuclease-free water. Samples should be submitted in an eppendorf tube clearly marked with a 2- to 3- digit identifier.

Library type

Input

Total required
gDNA DNA 1 µg

Output

Chip

Expected output 100bp reads

Expected output 200bp reads Number of useable reads (Millions)
314 >10 Mb >20 Mb 0.1-0.9
316 >100Mb >200 Mb 1.0-2.0
318 >500Mb >1 Gb 5.0-8.0