Genome Sequencing & Analysis Core Resource

Roche 454 GS-FLX Titanium and FLX+

We offer services on two Roche 454 GS-FLX+ sequencinger. 454 Sequencing is a long-read platform.This technology is often used for these applications: De novo genome or transcriptome sequencing, amplicon sequencing, genome or transcriptome resequencing, and metagenomics. It can produce more than a million reads at an >550bp average length for genome and transcriptome sequencing (FLX+ chemistry) and >375bp average length for amplicon and paired end sequencing (Titanium chemistry).

Sample requirements

Samples must be resuspended in TE or nuclease-free water. Samples should be submitted in an eppendorf tube clearly marked with a 2- to 3- digit identifier. See input amount in the table below.